In the present study, piperine was evaluated for its antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Alternaria alternata, Aspergillus niger, Aspergillus flavus, and Fusarium oxysporum. The antibacterial activity was measured by agar well diffusion method and antifungal activity by poisoned food technique. Piperine showed antimicrobial activity against all tested bacteria with zone of inhibition ranged from 8-18mm. maximum zone of inhibition was against Gram-positive bacteria Staphylococcus aureus (18mm) and minimum against Gram-negative bacteria Escherichia coli (8mm). Piperine showed maximum antifungal activity towards Fusarium oxysporum (14mm) and very least effect against Aspergillus niger (38mm). The results showed significant activity of piperine and suggesting its use as a natural antimicrobial agent.
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